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KMID : 0376219640010020235
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Chonnam Medical Journal
1964 Volume.1 No. 2 p.235 ~ p.245
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The Activity of Sorbitol Dehydrogenase and Motabolism of Sorbitol in Carbon Tetrachloride Poisoned Rabbits
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Abstract
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1. Changes in the activity of liver and serum sorbitol dehydrogenase (SDH) together with the metabolism of sorbitol were studied in acutely and chronically carbon tetrachloride poisined rabbits, and evidences were presented indicationg that acute and chronic form of liver damage can be easily detected by the deterination of serum SDH activity and by carrying out a sorbitol tolerance test.
2. The SDH was most abundantly found in the liver, and in liver localized exclusively at the cytoplasmic fraction, In nomal serum only negligible activity was detected.
3. The enzyme activity increased within a few hours after the injection of carbon tetrachloride. The highest activity was reached after 24 hours, and there after the enzyme level decreased sharply, although the decreasing rate was not so great as found in serum activity of glutamic-pyruvic transaminase(GPT).
4. At 50 hours after CC1₄poisoning, a considerable amount of SDH activity was still found to remain in the liver. However, in chronically CC1₄poisoned animals (CC1₄given for one month every other day), only negligible activity was found bothe in liver and in serum.
5. After injecting intravenously the supernatant fraction of normal liver as an enzyme source, the serum-activities of both SDH and GPT decreased rapidly until about 5hours and thereafter slowly. When a small amount of the enzyme source was given, the decreasing rate of serum GPT activity was found to be much delayed, indicating that decreasing patterns of an enzyme in serum is at least in part dependent on the initial enzyme level.
6. The intravenous injection on D-sorbitol in normals caused a rapid appearance in blood of fructose. The highest level of fructose was reached after 10minutes, and thereafter decreased rapidly, Essentially similar pattern was observed in acutely CC1₄poisoned animals, By contrast, chronically CC1₄poisoned rabbits showed significant decreases in the formation of fructose from D-sorbitol as well as in the utilization of the once formed fructose in blood.
7. Strangely enough, however, when fructose was administered intravenously from outside, even chronically CC1₄poisoned rabbits utilized equally well as normal, as evidenced by gructose tolerance test.
8. From these results, it was inferred that acute and chronic form of liver damate could be differentiated relatively easily on the basis of an elevated activity of serum SDH and of a disturbed sorbitol tolerance test, respectively.
Possible mechanisms for the differnet utilization of fructose observed in sorbito and fructose tolerance tests for chronically CC1₄poisoned rabbits have also been discussed in the light of metabolic controlling mechanisms.
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